Journal: International Journal of Genomics

Article Title: Integrative Single‐Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma

doi: 10.1155/ijog/1407947

Figure Lengend Snippet: Integrated single‐cell landscape of human osteosarcoma. (A) Harmony‐integrated UMAP of 39,063 quality‐controlled cells colored by global cluster. (B) The same embedding colored by patient, showing cross‐patient mixing after integration. (C) Reviewed major‐cell‐type annotations displayed on the integrated UMAP; labels mark lineage centroids. (D) Relative abundance of major cell types in each patient. Colors are consistent between panels C and D.

Article Snippet: HOS and U2OS human osteosarcoma cell lines were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, United States; HOS, CRL‐1543; U2OS, HTB‐96).

Techniques: Single Cell

Journal: International Journal of Genomics

Article Title: Integrative Single‐Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma

doi: 10.1155/ijog/1407947

Figure Lengend Snippet: TROAP depletion reduces clonogenic growth, overall wound closure, migration, and invasion in osteosarcoma cells. (A) Immunoblot analysis of TROAP in HOS and U2OS cells transfected with siNC, siTROAP‐1, or siTROAP‐2; beta‐actin is the loading control. (B) Colony‐formation images and colony counts. (C) Scratch‐wound images at 0 and 24 h and overall wound closure. Because the assay was not proliferation‐matched, it is not interpreted as direct migration evidence. (D) Transwell migration and invasion images and cell counts. Data are mean + /−standard deviation from three independent biological experiments. One‐way ANOVA with Dunnett′s test compared each siTROAP group with siNC; ∗∗∗ p < 0.001.

Article Snippet: HOS and U2OS human osteosarcoma cell lines were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, United States; HOS, CRL‐1543; U2OS, HTB‐96).

Techniques: Migration, Western Blot, Analysis, Transfection, Control, Colony Assay, Standard Deviation

Journal: Genes & Development

Article Title: Temporal gating dictates stress-induced transcript export from the nucleus

doi: 10.1101/gad.353896.126

Figure Lengend Snippet: HSPA1A accumulates in the nucleus in arsenite and heat stress. Subcellular localization of HSPA1A ( A ), poly(A) + RNAs ( B ), GAPDH ( C ), and NEAT1 ( D ) before, during, and after arsenite stress (“As,” 250 µM; 45 or 105 min), and HSPA1A ( E ) before, during, and after heat stress (“HS,” 45 or 105 min at 43°C). ( Left ) Representative smFISH images of U-2 OS cells under unstressed (“Un”), stressed, and recovery (“Rec,” 60 or 120 min poststress) conditions; nuclei in blue and RNA in white. Scale bars, 10 µm. ( Right ) Quantification of images from n = 3 independent experiments, with mean ± SEM of the percent nuclear RNA per cell shown (replicate means shown as diamonds). Forty-six to 132 cells were analyzed for each condition. Statistical significance shown using one-way ANOVA tests. (*) P < 0.05, (***) P < 0.005, (****) P < 0.001.

Article Snippet: U-2 OS cells (female osteosarcoma) purchased from ATCC were maintained in Dulbecco's modified Eagle medium (DMEM; Fisher Scientific) with 9% FetalGro EX (Rocky Mountain Biologicals), 1% penicillin/streptomycin (Gibco), and 1% Glutamax (Gibco) at 37°C and 5% CO 2 .

Techniques: